Experimental study of laboratory-heated CM2 chondrites Mighei and Murchison

We conducted experimental heating of two CM2 chondrites, Murchison and Mighei, to study changes in their oxygen isotopic compositions and mineralogy and explore possible genetic relationships between MCCs and normal CMs

The Galois Complexity of Graph Drawing: Why Numerical Solutions are Ubiquitous for Force-Directed, Spectral, and Circle Packing Drawings

Many well-known graph drawing techniques, including force directed drawings, spectral graph layouts, multidimensional scaling, and circle packings, have algebraic formulations. However, practical methods for producing such drawings ubiquitously use iterative numerical approximations rather than constructing and then solving algebraic expressions representing their exact solutions. To explain this phenomenon, we use Galois theory to show that many variants of these problems have solutions that cannot be expressed by nested radicals or nested roots of low-degree polynomials. Hence, such solutions cannot be computed exactly even in extended computational models that include such operations.Comment: Graph Drawing 201

In vitro and in vivo models to investigate tissue remodelling in Graves' ophthalmopathy.

In chapter one the aim was to establish and extend the characterization of murine models of thyroiditis and Graves' ophthalmopathy (GO), induced by transfer of TSH receptor (TSHR) primed T cells. In the first experiment, genetic immunization or TSHR fusion protein induced TSHR antibodies in all nine mice. Some of the antibodies functioned as thyroid-stimulating antibodies and/or TSH binding inhibiting Igs with 2/7 mice having elevated T4. Splenocyte transfer induced no immune response in naive BALB/cbyJico recipients. Subsequently genetic immunization or fusion protein-treated mice were maintained in either local or Brussels conditions (water, chow, and bedding). TSHR antibodies were induced in 9/9 Brussels (with decreased T4 in 1/9) but 5/9 local mice. No thyroiditis or orbital changes were induced, but misleading fixation artefacts in extraocular muscles were noted. Thyroids from many TSHR-treated and control mice contained ectopic thymus. Ectopic thymus can masquerade as thyroiditis, and care is required to avoid muscle artefacts. In chapter 2 the biological effects of TSHR activation in vitro, in adipose tissue - the site of orbital TSHR expression was examined. Activating mutant TSHR (TSHR*) or wild type (WT), were introduced into human orbital preadipocytes using retroviral vectors. Their proliferation, basal cAMP accumulation, spontaneous and PPARy induced adipogenesis were assessed and compared with non-modified cells. Expression of TSHR* significantly inhibited the proliferation of preadipocytes and produced an increase in unstimulated cAMP. PPARy induced adipogenesis in non-modified cells produced morphological changes, but not in TSHR* expressing cells. TSHR activation renders preadipocytes refractory to PPARy induced adipogenesis. The failure to develop lipid-containing vacuoles suggested the terminal stages of differentiation have been inhibited. In chapter 3 tears from patients with Graves' disease (GD), GO and smokers were examined for TSHR activation and protein changes. Reflex tears were collected from 37 normal non-smokers (NNS), 33 normal smokers (NS), 51 GD and 85 GO patients. Specific thyrotropin receptor stimulating activity (TSHRSA) was measured and also serum thyroid stimulating antibodies (TSAB). Pooled NNS, NS and GO tears were separated by SDS- PAGE and silver stained. Proteins expressed in the NS and GO pools but not NNS, were excised and analysed by MALDI-TOF mass spectrometry. Specific TSHRA was detected in 25% NS, 32% GD and 41% GO. A thyroid blocking antibody did not inhibit TSHRA. Clinical activity score correlated with serum TSAB but not tear TSHRSA. SDS-PAGE analysis revealed a higher vol/vol protein concentration in GO tears and additional proteins of 30 to 41 kD, in GO and NS compared with NNS. These were identified as zinc-alpha-2-glycoprotein (ZAG) and lactoferrin (LF). ZAG (also known as lipid mobilising factor) and LF do not have TSHRSA. Therefore, similar changes in tear composition are present in GO and NS compared with NNS. We postulate that TSHRSA is a protease enzyme. ZAG and LF expression is increased and their molecular weights are modified, suggesting degradation and/or changes in glycosylation, which may affect their bioactivities. In chapter 4, transgenic adiponectin elevation in FVB mice produced profound changes in interscapular and orbital fat depots. No previous models have demonstrated orbital fat proliferation and these changes could be followed by orbital MRI. TSHR expression increases with adipogenesis but does not in this case lead to autoimmunity directed towards the TSHR. A wide range of circulating adiponectin levels are present GO patients and no clinical correlations were evident

Transitions to Proton States in the 90-Zr(p,p') Reaction at 160 MeV

This work was supported by National Science Foundation Grant PHY 76-84033 and Indiana Universit

Spin-Orbit Effects on the Shapes of Cross Sections in the 90-Zr(p,p') Reaction at 160 MeV

This work was supported by National Science Foundation Grants PHY 76-84033A01, PHY 78-22774, and Indiana Universit